No One Can Truly Measure Exosomes: The Truth About Exosome Quantification

8 hours ago

Measuring exosomes remains an imperfect science.
Exosomes are extremely small extracellular vesicles measuring approximately 30–150 nanometers. Current technologies estimate particle size and concentration indirectly, making results highly dependent on instrument settings and analytical methods.
Production and purification significantly affect exosome quality.
Factors such as oxygen levels, temperature, pH, cellular stress, and stem cell age influence exosome production. Proper filtration techniques are essential to remove larger particles and enrich for true exosomes.
Biological activity may matter more than particle count.
Dr. Park argues that measuring the proteins, RNA, and biological signaling components inside exosomes may provide a more meaningful assessment of product quality than simply counting particles.

Ed Park, MD

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Ed Park, MD

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